Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.
Open Access
- 1 June 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 73 (3), 783-788
- https://doi.org/10.1083/jcb.73.3.783
Abstract
A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.Keywords
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