Sequence-specific RNA binding by the HIV-1 Rev protein
- 7 December 1989
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 342 (6250), 714-716
- https://doi.org/10.1038/342714a0
Abstract
THE human immunodeficiency virus type 1 (HIV-1) Rev protein acts post-transcriptionally to increase the amounts of the viral gag-pol and env messenger RNAs in the cytoplasm of infected cells1,2. The mechanism of Rev action is uncertain. Possibilities include an accelerating effect on the rate of export of its mRNA targets from the nucleus3–6 and/or modulation of the splicing of pre-mRNAs (reviewed in ref. 7). Both the gag-pol and env mRNAs contain a sequence that is required for responsiveness to Rev—the Rev responsive element, RRE3,4,6,8. Here we show that Rev is a sequence-specific binding protein, whose binding site is the RRE. This information should help to clarify the mechanism by which Rev acts.Keywords
This publication has 23 references indexed in Scilit:
- RNA-binding proteins as developmental regulators.Genes & Development, 1989
- A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP proteinCell, 1989
- The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNANature, 1989
- Revving up gene expressionNature, 1989
- Functional replacement of the HIV-1 rev protein by the HTLV-1 rex proteinNature, 1988
- Immunodeficiency virus rev trans-activator modulates the expression of the viral regulatory genesNature, 1988
- GAL4 derivatives function alone and synergistically with mammalian activators in vitroCell, 1988
- Purification and Biochemical Characterization of the Promoter-Specific Transcription Factor, Sp1Science, 1986
- HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNACell, 1986
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985