Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase
Open Access
- 15 September 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (18), 6590-6595
- https://doi.org/10.1128/jb.187.18.6590-6595.2005
Abstract
By taking advantage of the tightly clustered genes for the membrane-bound [NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein.Keywords
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