Characterization of Feline Whole-blood Cultures and Determination of the Frequency of Radiation-induced Dicentrics in Human and Feline Lymphocytes

Abstract
The Ham's F-10 and PHA culture system was applied to whole feline blood, and cell-cycle characteristics such as DNA synthesis and mitotic indices were studied. The results are comparable to those obtained from human whole-blood cultures. The yields of dicentrics were also determined in lymphocytes from X-irradiated human and feline blood. The ratio between the experimental yields of dicentrics in human as compared to feline lymphocytes was 1:0·27.