Kinetic studies of Escherichia coli transfer RNA (uracil-5-)-methyltransferase
- 1 March 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (6), 1068-1072
- https://doi.org/10.1021/bi00599a020
Abstract
The kinetic mechanism of a semipurified tRNA (uracil-5-)-methyltransferase (EC 2.1.1.35) preparation obtained from E. coli was studied at pH 9.0 in the presence and absence of products. The initial velocity and product inhibition patterns are consistent with a random order of addition of adenosylmethionine and tRNA to separate and independent binding sites on the enzyme. Values were determined for the Km and product inhibitor constants.This publication has 11 references indexed in Scilit:
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