Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method

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Abstract
A method for the analysis of IAA in plant extracts was developed based on high performance liquid chromatography separation of IAA on a microparticulate strong anion exchange column followed by quantitation with 2 selective detectors: an electrochemical, carbon paste amperometric detector and/or a fluorescence detector. The detection limit for IAA is less than 1 ng with the fluorescence detector and less than 50 pg with the electrochemical detector. The IAA levels are reported for various tissues of wheat [Triticum aestivum], pinto beans [Phaseolus vulgaris], soybeans [Glycine max], cotton [Gossypium hirsutum] and corn [Zea mays].