A CLINICAL METHOD FOR THE DETERMINATION OF URINARY PREGNANEDIOL AND PREGNANETRIOL

Abstract

The method involves glucuronidase hydrolysis, extraction, and chromatography on disposable 1-g silica gel columns. The specificity of the pregnanediol determination is increased by acetylation and rechromatography on the same type of column. Sensitivity of the method is doubled by the use of sulfuric acid saturated with SO2 gas as the chromogenic agent.