Repair of a synthetic abasic site in DNA in a Xenopus laevis oocyte extract.
Open Access
- 1 September 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (9), 3750-3757
- https://doi.org/10.1128/mcb.9.9.3750
Abstract
Covalently closed circular DNA containing a synthetic analog of an abasic site at a unique position was used as a substrate to study DNA repair. Incubation of this DNA in Xenopus laevis oocyte extracts resulted in rapid cleavage of the DNA at the abasic site by a class II apurinic-apyrimidinic endonuclease, followed by complete repair within 40 min. Nicked circular DNAs persisted for several minutes before repair by an ATP-dependent DNA synthesis reaction. The repair-related DNA synthesis was localized within 3 or 4 nucleotides surrounding the abasic site. These results are consistent with the short-patch repair reported for DNA damage at heterogeneous sites in human cells (J. D. Regan and R. B. Setlow, Cancer Res. 34:3318-3325, 1974).This publication has 28 references indexed in Scilit:
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