Rapid High-Performance Liquid Chromatographic Method for the Analysis of Debrisoquine and 4-Hydroxydebrisoquine in Urine without Derivatization
- 28 February 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (5), 961-968
- https://doi.org/10.1080/01483919008049225
Abstract
A simple and rapid HPLC method has been developed for the simultaneous determination of debrisoquine (D) and its main metabolite, 4-hydroxydebrisoquine (4OHD) in urine, without derivatization of the compounds. Guanoxan is used as the internal standard. The chromatographic separation was accomplished with a mobile phase comprising 15% acetonitrile and 85% 8 mM phosphate buffer (pH 5) at a flow rate of 1 ml/min. The HPLC column was packed with 5 μm Spherisorb-CN. The eluant was monitored at 214 nm. The intra- and inter-assay coefficients of variation were less than 6%. The lowest limit of detection for D was 0.1 μg/ml and for 4OHD was 0.05 μg/ml. The method is suitable for determining the oxidation phenotype of populations and is presently being used to study the oxidation polymorphism in Maori and South Pacific Polynesians.This publication has 8 references indexed in Scilit:
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