The Effect of Dietary Fat or Cholesterol and Cholic Acid on the Rate of Synthesis of Rat Liver Glucose-6-P Dehydrogenase
- 1 October 1978
- journal article
- research article
- Published by Elsevier in Journal of Nutrition
- Vol. 108 (10), 1708-1717
- https://doi.org/10.1093/jn/108.10.1708
Abstract
The effect of dietary fat or cholesterol and cholic acid on the rate of synthesis of rat liver glucose-6-P dehydrogenase was measured by isolating hepatocytes from the livers of rats fed diets containing various amounts and types of lipid. Hepatocyte proteins were labeled with [3H]-leucine and glucose-6-P dehydrogenase was immunoprecipitated with a specific antiserum. The immunoprecipitates were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels and the radioactivity in the glucose-6-P dehydrogenase subunit was used to calculate the relative rate of synthesis of the enzyme. It is concluded that the activity of glucose-6-P dehydrogenase is primarily regulated by alterations in the rate at which the enzyme is synthesized. Dietary fat may decrease the rate of degradation of glucose-6-P dehydrogenase.Keywords
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