Application of Trifluoroacetyl Derivatives to Sugar and Lipid Chemistry: I. Gas Chromatographic Analysis of Common Constituents of Glycolipids

Abstract

A method is described to determine gaschromatographically the individual component of glycosphingolipid after methanolysis and trifluoroacetylation. By using a mixed coated column of SE-30 and XE-60 and under suitable temperature-programmed conditions, neutral hexoses, hexosamines, neuraminic acid, and sphingosine bases from glycolipids were analyzed in one run with considerable accuracy.