Die Einwirkung von Giften auf die Atmung von Backhefe, ihr Nachweis durch die elektrochemische O2-Messung

Abstract

The following compounds were added to respiring baker''s yeast; potassium cyanide, sodium azide, sodium sulfide, hydroxylamine hydrochloride, nicotinic acid amide, ascorbic acid, sodium barbiturate, malonic acid, sodium fluoride, bromacetic acid, trichloracetic acid, potassium thiocyanate, and penicillin-G sodium salt. The concentration of the poison in the solution was 10-2 mol/l and less, to cessation of toxic action. None of the reagents named caused a stimulation of respiration similar to that found in Chlorella. A strong inhibition occurred only with weak electrolytes. Hydrocyanic acid, generally considered very toxic, was exceeded in toxicity by hydrogen sulfide tenfold and by azide fivefold. Hydroxylamine required a longer time for the development of full toxicity than hydrocyanic acid, hydrogen sulfide, and hydrazide. Potassium cyanide, sodium azide, sodium sulfide, hydroxylamine hydrochloride, and penicillin had no effect on the electrochemical measurement of oxygen.