Establishment, characterization and virus expression of cell lines derived from radiation‐ and virus‐induced lymphomas of C57BL/Ka mice

Abstract

Permanent cell lines have been established in vitro from lymphoid tumors induced in C57BL/Ka mice by fractionated X-irradiation or by inoculation of the radiation leukemia virus (RadLV). The cultured cells are lymphoblastic, replicate rapidly in vitro, and are tumorigenic in vivo. The cell surface markers Thy 1, Ly 1, Ly 2,3 and Gix are expressed by the lymphoid tumor cells in the mouse and persist in the corresponding cell lines; expression of the H-2 and TL antigens is greatly reduced during in vitro passage, but is restored on in vivo transplantation. The cell lines derived from RadLV-induced tumors (BL/VL lines) produce a virus population (RadLV/LTC) with the thymotropic and leukemogenic attributes of RadLV. Those derived from radiation-induced, virus-negative lymphomas (BL/RL lines) are initially devoid of MuLV expression, but frequently become spontaneous virus producers during in vitro cultivation.