Chiral Monolithic Columns for Enantioselective Capillary Electrochromatography Prepared by Copolymerization of a Monomer with Quinidine Functionality. 2. Effect of Chromatographic Conditions on the Chiral Separations

Abstract

The effect of chromatographic conditions on the performance of chiral monolithic poly(O-[2-(methacryloyloxy)ethylcarbamoyl]-10,11-dihydroquinidine-co-ethylene dimethacrylate-co-2-hydroxyethyl methacrylate) columns in the capillary electrochromatography of enantiomers has been studied. The flow velocity was found to be proportional to the pore size of the monolith and both the pH and the composition of the mobile phase. The length of both open and monolithic segments of the capillary column was found to exert a substantial effect on the run times. The use of monoliths as short as 8.5 cm and the “short-end” injection technique enabled the separations to be achieved in ∼5 min despite the high retentitivity of the quinidine selector. Very high column efficiencies of close to 250 000 plates/m and good selectivities were achieved for the separations of numerous enantiomers using the chiral monolithic capillaries with the optimized chromatographic conditions.