Sphingomyelin-enriched microdomains define the efficiency of native Ca2+-triggered membrane fusion
Open Access
- 1 July 2006
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 119 (13), 2688-2694
- https://doi.org/10.1242/jcs.03007
Abstract
Membrane microdomains or `rafts' are suggested to act as regulators of the exocytotic process and also appear to be the sites of Ca2+-triggered membrane fusion. Microdomains are postulated to maintain the localization of `efficiency' factors, including Ca2+ sensors and other protein and lipid components. Separation of the fundamental ability to fuse from the efficiency of the process has suggested dependence of efficiency factors on microdomain organization. Cholesterol, a key component of membrane microdomains, contributes to both the efficiency and the fundamental ability to fuse. However, testing for a selective effect of native microdomains on the efficiency of fusion, without affecting membrane cholesterol density, has not been assessed. Hydrolysis of sphingomyelin disrupts native raft domains on secretory vesicles. Disruption of microdomains enriched in sphingomyelin-cholesterol by treatment with sphingomyelinase selectively and dose dependently inhibited the Ca2+ sensitivity and late kinetics of secretory vesicle fusion. As a native microdomain constituent, sphingomyelin is associated with Ca2+ sensing through its interaction with other raft-bound lipid and/or protein factors, thereby supporting the physiological Ca2+ sensitivity of membrane fusion. Furthermore, the sphingomyelinase-driven generation of ceramide, contributing to the total membrane negative curvature, preserves the ability to fuse despite extensive cholesterol removal. Membrane microdomain integrity thus underlies the efficiency of fusion but not the fundamental ability of native vesicles to undergo Ca2+-triggered membrane merger. The results are consistent with a fundamental fusion machine of intrinsically low Ca2+ sensitivity that, supported by accessory `efficiency' components, facilitates Ca2+-triggered bilayer merger under physiological conditions.Keywords
This publication has 41 references indexed in Scilit:
- Cholesterol facilitates the native mechanism of Ca2+-triggered membrane fusionJournal of Cell Science, 2005
- Asymmetric Addition of Ceramides but not Dihydroceramides Promotes Transbilayer (Flip-Flop) Lipid Motion in MembranesBiophysical Journal, 2005
- Regulated secretion: SNARE density, vesicle fusion and calcium dependenceJournal of Cell Science, 2003
- Role of sphingomyelinase and ceramide in modulating rafts: do biophysical properties determine biologic outcome?FEBS Letters, 2002
- SNARE proteins are highly enriched in lipid rafts in PC12 cells: Implications for the spatial control of exocytosisProceedings of the National Academy of Sciences, 2001
- Structure and Function of Sphingolipid- and Cholesterol-rich Membrane RaftsJournal of Biological Chemistry, 2000
- Biochemical and Functional Studies of Cortical Vesicle Fusion: The SNARE Complex and Ca2+ SensitivityThe Journal of cell biology, 1998
- The influence of cholesterol on phospholipid membrane curvature and bending elasticityBiophysical Journal, 1997
- Effects of cholesterol on the structural transitions induced by diacylglycerol in phosphatidylcholine and phosphatidylethanolamine bilayer systemsBiochemistry and Cell Biology, 1990
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959