Location and Function of fruC, a Gene Involved in the Regulation of Fructose Utilization by Escherichia coli
- 1 February 1987
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 133 (2), 341-346
- https://doi.org/10.1099/00221287-133-2-341
Abstract
Procedures are described for the selection of Escherichia coli mutants that constitutively take up and phosphorylate fructose, and convert it to fructose 1,6-bisphosphate. The phenotype of such mutants is described. The altered regulatory gene, fruC, is highly co-transducible with leu and other markers located at min 2 on the genome. In merozygotes, fruc+ is dominant to fruC. Mutants can be readily isolated that are fruC at 42.degree. C but fruC+ at 30.degree. C; moreover, the integration of a Tn10 transposon in the genome at min 2 converts fruC+ strains to fruC. It is therefore likely that the FruC+ regulatory gene specifies a repressor protein.This publication has 11 references indexed in Scilit:
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