Eukaryotic DNA methylases and their use forin vitromethylation
- 30 January 1990
- journal article
- research article
- Published by The Royal Society in Philosophical Transactions of the Royal Society of London. B, Biological Sciences
- Vol. 326 (1235), 189-198
- https://doi.org/10.1098/rstb.1990.0003
Abstract
DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, which makes it difficult to saturate all possible sites on the DNA, but procedures are described that circumvent this problem.This publication has 33 references indexed in Scilit:
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