Composition of fluid obtained from choroid plexus tissue isolated in a chamber in situ*
- 1 December 1972
- journal article
- Published by Wiley in The Journal of Physiology
- Vol. 227 (1), 127-139
- https://doi.org/10.1113/jphysiol.1972.sp010023
Abstract
1. A method was developed for isolating a segment of the choroid plexus of the lateral ventricle of the cat brain in a chamber in situ. 2. A comparison of electrolyte and protein concentrations in serum, ultrafiltrate of serum, cisterna magna fluid, fluid accumulated in the chamber and fluid collected from the choroid plexus by another technique, demonstrates that the chamber fluid is a secretory product of the choroid plexus. 3. The rate of fluid formation in the chamber was 0·4 μl. min−1 mg−1 of tissue, a value in good agreement with reports in the literature. 4. The observation that the concentration of K+ in choroid plexus fluid was lower than that in the serum ultrafiltrate suggests that K+ is regulated by an active transport process at the choroid plexus. 5. Significant correlation was found between electrolyte values and the protein content of the chamber fluid. This suggests that plasma is the probable source of the protein in the chamber fluid and that K+ and probably Ca2+ and Mg2+ in c.s.f. are subject to active homoeostatic regulation by the choroid plexus. 6. The technique described provides a new opportunity to study in detail the functional characteristics of the choroid plexus.Keywords
This publication has 15 references indexed in Scilit:
- Cerebrospinal Fluid Production by the Choroid Plexus and BrainScience, 1971
- Physiology of the choroid plexus.Physiological Reviews, 1971
- Effect of magnesium deficiency on electrolyte distribution in the rabbitAmerican Journal of Physiology-Legacy Content, 1969
- Magnesium metabolismErgebnisse der Physiologie Biologischen Chemie und Experimentellen Pharmakologie, 1967
- Secretion of cerebrospinal fluid by the ventricular ependyma of the rabbitAmerican Journal of Physiology-Legacy Content, 1967
- Extra-ventricular formation of cerebrospinal fluid.1967
- Effects of Pco2 acetazolamide and ouabain on volume and composition of choroid‐plexus fluid.The Journal of Physiology, 1965
- Relation of potassium concentration in choroidplexus fluid to that in plasma.The Journal of Physiology, 1965
- An instrument and method for automatic, rapid, accurate, and sensitive titration of chloride in biologic samples.1958
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951