Bull semen was collected artifically and vitamin A was determined in the intact spermatozoa, the acrosomes and the sperm tails. The results show that over 90% of the total vitamin A in the spermatozoa was present in the acrosomes and that the sperm tails were completely devoid of vitamin A. The concentration of vitamin A varied from 224 to 364 ng/10-9 spermatozoa.