Effect of polyvalent metal ions on the reactivity of human O6-methylguanine-DNA methyltransferase
- 1 April 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 9 (4), 683-685
- https://doi.org/10.1093/carcin/9.4.683
Abstract
Polyvalent metal Ions are highly effective in inhibiting human O6 -methylguanine-DNA methyltransferase, the repair protein responsible for the removal of the promutagenic and presumably procarcinogenic adduct, O6 in DNA. The sulfhydryl group-reacting metal ions (Cd 2+ , Zn 2+ , Hg 2+ ) completely inhibited the reaction at concentrations of 100–l500 μM while other metal ions (Al 3+ , Fe 3+ ) required concentrations of 1mM or greater for significant reduction of the reaction rate. Inhibition by the former group of metals could be reversed by dithiothreitol but not by EDTA, while the opposite was true for the second group. Under conditions of partial Inhibition of the initial reaction rate by either Hg 2+ or Al 3+ the extent of reaction was not significantly affected, indicating reversible binding of these ions.This publication has 2 references indexed in Scilit:
- Purification and some properties of human DNA-O6-methylguanine methyltransferaseJournal of Biosciences, 1987
- Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase.Journal of Biological Chemistry, 1985