Defective binding of IRFs to the initiator element of interleukin‐1β‐converting enzyme (ICE) promoter in an interferon‐resistant Daudi subline
- 7 May 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 450 (3), 263-267
- https://doi.org/10.1016/s0014-5793(99)00515-3
Abstract
To investigate mechanisms of interferon (IFN) resistance, we have established an IFN-resistant Daudi subline (Daudires), which is 1×104 times more resistant to IFN-α than parental cells. Among the IFN-inducible genes examined, only ICE mRNA expression was deficient in Daudires cells. We then analyzed the regulatory mechanisms of ICE transcription, and found that IFN-induced activation of the ICE promoter was dependent on the binding of IRFs to its initiator (Inr) element. Inr binding of IRFs was markedly diminished in Daudires cells, and forced expression of IRF-1 was able to activate the ICE promoter to the level of parental cells. These results suggest that IRFs and their target genes, as represented by ICE in this study, are involved in IFN resistance.Keywords
This publication has 28 references indexed in Scilit:
- Interferon-resistant Human Melanoma Cells Are Deficient in ISGF3 Components, STAT1, STAT2, and p48-ISGF3γPublished by Elsevier ,1997
- Modulation of E2F Activity Is Linked to Interferon-induced Growth Suppression of Hematopoietic CellsPublished by Elsevier ,1997
- Antiproliferative effects of interferon alpha on human pancreatic carcinoma cell lines are associated with differential regulation of protein kinase C isoenzymes.Gut, 1996
- Effects of Interferons and Tumour Necrosis Factor-α on Human Lung Cancer Cell Lines and the Development of an Interferon-Resistant Lung Cancer Cell LineActa Oncologica, 1996
- An IRF-1-dependent pathway of DNA damage-induced apoptosis in mitogen-activated T lymphocytesNature, 1995
- Molecular Characterization of the Gene for Human Interleukin-1β Converting Enzyme (IL1BC)Genomics, 1994
- TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNACell, 1994
- Reversal of Interferon-γ-Resistant Phenotype by Poly(I:C): Possible Involvement of ISGF2 (IRF1) in Interferon-γ-Mediated Induction of the IDO GeneJournal of Interferon Research, 1994
- An alternative pathway for transcription initiation involving TFII-INature, 1993
- A general method for rapid site-directed mutagenesis using the polymerase chain reactionGene, 1990