Cryptosporidium parvum Mixed Genotypes Detected by PCR-Restriction Fragment Length Polymorphism Analysis
Open Access
- 1 January 2002
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (1), 427-429
- https://doi.org/10.1128/aem.68.1.427-429.2002
Abstract
Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.Keywords
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