THE SITE OF BINDING OF PYRIDOXAL-5′-PHOSPHATE TO HEAR GLUTAMIC-ASPARTIC TRANSAMINASE
- 1 September 1962
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 48 (9), 1615-1618
- https://doi.org/10.1073/pnas.48.9.1615
Abstract
A tetradecapeptide containing covalently bound pyridoxyl-5[image]-phosphate has been isolated from pig heart glutamic-aspartic transaminase after reduction with sodium borohydride and subsequent degradation with chymotrypsin. A partial structure for the peptide has been shown to be Ser. Thr. Glu. (Asp, Gly, Ala, Val. Ileu, [epsilon]-pyridoxyllys, Lys) Gly. Ser. Asp. Phe. The significance of the results, together with similar information as to the site of binding of the vitamin derivative to muscle phosphorylase [alpha], is discussed.Keywords
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