Crystalline Cytochrome C

Abstract

Details of the methods used for crystallization of cytochrome c from many animal muscles and beef kidney have been described in the preceding four reports (1-4). The method employed for crystallization from bakers' yeast was also reported (5). Yakushiji (6) and Goddard (7) purified cytochrome c from a laver (Porphyra tenera) and wheat germ respectively. Purification from wheat germ was not easy due to the low content of cytochrome c, to difficulty in its extraction and also to the presence of many impurities having similar adsorption characteristic to cytochrome c. However, by the improved extraction method and careful treatment with the resin, the pigment was crystallized. This was already reported as a short communication (8). In this report the details of the method and its results will be reported.