The flame-spectrophotometric determination of calcium in biological fluids and an isotopic analysis of the errors in the Kramer–Tisdall procedure

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Abstract
A flame-spectro-photometric method of estimating calcium in biological fluids is described. Spectral interference due to sodium is mostly eliminated by measuring the calcium emission at 423 m[mu]. Phosphate interference is overcome by diluting the samples with excess of phosphate. Studies with (Ca45) show that about 7% of calcium in serum is lost during washing and precipitation in the procedure of Kramer and Tisdall (Tisdall, 1923). Magnesium is coprecipitated in this procedure in a variable amount (range 0-11%), averaging 3%. It is concluded that the accepted normal serum-calcium figures are approximately 4% too low.