Crosslinking of actin filaments is caused by caldesmon aggregates, but not by its dimers
- 11 March 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 182 (1), 201-204
- https://doi.org/10.1016/0014-5793(85)81184-4
Abstract
A recent report by Bretscher [(1984) J. Biol. Chem. 259, 12873-12880] showed that caldesmon prepared by his method crosslinks actin filaments to form thick bundles. This is in contrast to the results of previous work that caldesmon binds to F-actin but does not cause any gelation [(1981) Proc. Natl. Acad. Sci. USA 78, 5652-5655]. The present work clearly showed that caldesmon purified according to Bretscher does not cause any gelation of F-actin. However, caldesmon aggregates formed by concentration or by freeze-thawing gelated F-actin to form bundles.CaldesmonActin-associated proteinCalmodulin-binding proteinActin-crosslinkerChicken gizzarKeywords
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