Isolation of Sperm Cells from the Pollen of Plumbago zeylanica
- 1 May 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 81 (1), 317-319
- https://doi.org/10.1104/pp.81.1.317
Abstract
Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 .times. 106 cells/milliliter. Such ''gametoplasts'' remain intact for up to 24 hours according to Evan''s blue exclusion and can be used for characterization or physiological manipulation.This publication has 5 references indexed in Scilit:
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- The Distribution and Phylogenetic Significance of Binucleate and Trinucleate Pollen Grains in the AngiospermsAmerican Journal of Botany, 1967