COMPOUNDS OF PHOSPHORUS IN THE HEART AND STRIATED MUSCLES OF THE DOG: METHODS OF DETERMINATION AND NORMAL VALUES

Abstract

Values are presented for the acid-soluble phosphate compounds of cardiac and skeletal tissue in the normal dog. Fraction A, which is not precipitated by Ba salts, is composed chiefly of phosphocreatine and hexose monophosphate, but there may be also a small amt. of a compound soluble in the presence of alcohol and Ba hydroxide. In cardiac tissue, the phosphocreatine may occur in amts. as high as 4 of that in skeletal tissue. Fraction B, which is precipitated by Ba salts, contains inorganic P and organic P, the latter largely adenylpyrophosphate; the significant amt. left to be accounted for probably consists of other mono-nucleotides. In both cardiac and skeletal tissues of the dog, this fraction produces a pos. reaction in Wheeler and Johnson''s color test for cytosine and uracil. Values for total phosphates are ca. the same in amt. in both skeletal and cardiac tissue. A larger proportion of the total phosphates in the skeletal tissue is acid-soluble.