Synthesis and characterization of [1‐13C]‐and d8‐arachidonic acid
- 1 September 1979
- Vol. 14 (9), 819-821
- https://doi.org/10.1007/bf02533522
Abstract
Methyld 8- and [1-13C] 5,8,11,14-eicosatetraenoate (arachidonate) were prepared from a common synthetic precursor, 4,7,10,13-nonadecatetrayn-1-ol. The purified products were characterized by gas chromatography-mass spectrometry. Mass spectra oft-butyldimethylsilyl esters ofd 8-and [1-13C]-arachidonic acid showed a most intense [M-57]+ peak at high mass. The isotopic purity of methyl [1-13C] arachidonate was 99% and that of methyld 8-arachidonate was 56%. Whend 8-arachidonic acid was prepared by direct deuteration of 5,8,11,14-eicosatetraynoic acid, the isotopic purity of the sample was 86%.Keywords
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