ECHO and Poliomyelitis Virus Antisera Prepared in Guinea Pigs with Fluorocarbon-Treated Cell Culture Antigens

Abstract

ECHO and poliomyelitis virus antisera without host CF antibodies can be prepared in guinea pigs by immunizing the animals with fluorocarbon-treated cell culture grown virus. Although in repeated immunization of guinea pigs 3 out of 12 fluorocarbon-treated antigens with no host CF activity produced host antibodies in titers of 1:32 and 1:64, specific virus titers were 16 and 32 times higher. Besides the host activity, the specific virus activity of fluorocarbon-treated antigens should be tested before using them for immunization.