RADIOMICROASSAY FOR CYTOTOXIC ANTIBODY TO HUMAN SPERMATOZOA - QUANTIFICATION BY TRITIATED ACTINOMYCIN-D
- 1 January 1977
- journal article
- research article
- Vol. 27 (3), 469-477
Abstract
The described assay uses [3H]actinomycin D (Act D) to label damaged spermatozoa in a fashion analogous to penetration by vital dye. Optimal conditions for and some kinetics of the assay are presented. The assay is sensitive, reliable, simple to perform and uses only small amounts of serum and spermatozoa. Applied to sperm antibody positive human postvasectomy sera, the assay compared favorably in sensitivity with vital dye microscopic observations and with parallel titration by the Isojima''s immobilization test.This publication has 9 references indexed in Scilit:
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