A Kinase That Transfers the γ-Phosphoryl Group of GTP to Proteins of Eukaryotic 40S Ribosomal Subunits
- 1 February 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (2), 350-354
- https://doi.org/10.1073/pnas.71.2.350
Abstract
An enzyme in rat-liver cytosol transferred the gamma-phosphoryl of GTP to serine and threonine residues of at least four proteins (S6, S10, S14 or S15, and S17) of the small (40S) subunit of rat-liver ribosomes. A number of nonribosomal proteins in the enzyme preparation were also phosphorylated; they were preferentially and tightly bound to the large subunit. The enzyme could be distinguished from protein kinase-ATP (which also phosphorylated ribosomal proteins) by a number of criteria: (1) GTP was the phosphoryl donor; (2) the pattern of phosphorylation of ribosomal proteins by the two enzymes was different; and (3) the protein kinase that used GTP as the phosphoryl donor was not stimulated by cyclic AMP (or by cyclic GMP).Keywords
This publication has 21 references indexed in Scilit:
- Proteins at the tRNA Binding Sites of Escherichia coli RibosomesProceedings of the National Academy of Sciences, 1974
- Determination of the Number of Proteins in Liver Ribosomes and Ribosomal Subunits by Two-Dimensional Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1972
- Phosphorylation of ribosomal proteins in rabbit reticulocytes. A cell-free system with ribosomal protein kinase activityBiochemistry, 1971
- Ribosomal proteins of Escherichia coli. I. Purification of the 30 S ribosomal proteinsBiochemistry, 1969
- Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats.Proceedings of the National Academy of Sciences, 1968
- Mammalian Ribosomal Protein: Analysis by Electrophoresis on Polyacrylamide GelScience, 1967
- Insulin regulation of protein synthesis by muscle ribosomes: effect of the hormone on translation of messenger RNA for a regulatory protein.Proceedings of the National Academy of Sciences, 1966
- Analysis of C14-labeled proteins by disc electrophoresisBiochemical and Biophysical Research Communications, 1965
- THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLIProceedings of the National Academy of Sciences, 1964
- A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activityBiochemical Journal, 1964