SecE‐dependent overproduction of SecY in Escherichia coli
- 20 August 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 269 (1), 96-100
- https://doi.org/10.1016/0014-5793(90)81128-b
Abstract
The secY and secE genes were individually cloned and placed under the control of the tac promoter on plasmids. Induction with isopropyl-β-D-thiogalactopyranoside resulted in the overproduction of SecE, but not that of SecY. The simultaneous induced expression of both genes in the same cells resulted in the overproduction of SecY together with that of SecE. SecY and SecE thus overproduced were localized in the cytoplasmic membrane as those expressed at the normal levels were. It is suggested that SecY and SecE interact with each other in the cytoplasmic membrane. The numbers of the SecY and SecE molecules per cell were estimated.Keywords
This publication has 28 references indexed in Scilit:
- PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coliCell, 1990
- The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.Genes & Development, 1989
- A new hybrid promoter and its expression vector in Escherichia coli.Agricultural and Biological Chemistry, 1988
- Overproduction, isolation and determination of the amino-terminal sequence of the SecY protein, a membrane protein involved in protein export in Escherichia coliEuropean Journal of Biochemistry, 1986
- A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteinsCell, 1983
- Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?Cell, 1981
- Suppressor mutations that restore export of a protein with a defective signal sequenceCell, 1981
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976
- Replication control in a composite plasmid constructed by in vitro linkage of two distinct repliconsNature, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970