Insulin and incorporation of radioactivity into nucleic acid fraction of isolated diaphragm

Abstract

Insulin in vitro stimulated the incorporation into the nucleic acid fraction of isolated rat diaphragm of radioactivity from d-glucose-U-C¹⁴, adenine-8-C¹⁴ and orotic acid-6-C¹⁴; insulin had no effect on the incorporation of thymine-2-C¹⁴ into muscle nucleic acids. Insulin enhanced the incorporation into nucleic acids of C¹⁴ from adenine and orotic acid in the absence of added glucose, and incorporation of adenine-8-C¹⁴ was not influenced by glucose concentration over the range 0–600 mg %.