Negative Regulation of CD45 Protein Tyrosine Phosphatase Activity by Ionomycin in T Cells
- 20 September 1991
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 253 (5026), 1423-1425
- https://doi.org/10.1126/science.1654595
Abstract
CD45 is a leukocyte-specific, transmembrane protein tyrosine phosphatase (PTPase) required for T cell responsiveness. How the activity of PTPases is regulated in vivo is unclear. Treatment of murine thymocytes and a variety of murine T cell lines with the calcium ionophore ionomycin decreased CD45 PTPase activity. Ionomycin treatment also led to a decreased phosphorylation of serine residues in CD45. These results indicate that increased intracellular calcium modulates CD45 PTPase activity, demonstrating regulation of CD45 PTPase activity in vivo, and also implicate serine dephosphorylation as a possible mechanism.Keywords
This publication has 27 references indexed in Scilit:
- Coclustering CD45 with CD4 or CD8 alters the phosphorylation and kinase activity of p56lck.The Journal of Experimental Medicine, 1990
- cAMP-DEPENDENT PROTEIN KINASE: FRAMEWORK FOR A DIVERSE FAMILY OF REGULATORY ENZYMESAnnual Review of Biochemistry, 1990
- Protein-tyrosine phosphatases: The other side of the coinCell, 1989
- THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASESAnnual Review of Biochemistry, 1989
- The Leukocyte Common Antigen FamilyAnnual Review of Immunology, 1989
- Demonstration that the leukocyte common antigen (CD45) is a protein tyrosine phosphataseBiochemistry, 1988
- Phorbol diesters increase the phosphorylation of the leukocyte common antigen CD45 in human T cellsEuropean Journal of Immunology, 1987
- Calcium transport by ionophorous peptides in dog and human lymphocytes detected by quin-2 fluorescenceBiochemical and Biophysical Research Communications, 1986
- Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol esterNature, 1985
- Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.The Journal of Experimental Medicine, 1978