Characterization of aminoglycoside 6'-N-acetyltrasferases [AAC(6')] from Gram-negative bacteria and Streptomyces kanamyceticus

Abstract

Aminoglycoside-6′-N-acetyltransferase [AAC(6′) enzymes] from several Gram-negative bacteria and Streptomyces kanamyceticus were purified by affinity and ion exchange chromatography. The different enzymes were characterized by their biochemical properties including molecular weights, isoelectricpoints, substrate ranges, and pH-optima. These biochemlcal characteristics were compared to the data for 6′-N-acetyltransferases from other Gram-negative and Gram-positive bacteria. All data indicated that the group of AAC(63 enzymes IS rather heterogeneous from the biochemical point of view and led us to a new proposal for classification of subgroups.