H-2 Homology Requirements for Secondary Cell-Mediated Lympholysis and Mixed Lymphocyte Reactions to TNP-Modified Syngeneic Lymphocytes
- 1 April 1977
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 118 (4), 1420-1427
- https://doi.org/10.4049/jimmunol.118.4.1420
Abstract
Secondary cell-mediated lympholysis (CML) and mixed lymphocyte reactions (MLR) were generated in a tissue culture system against trinitrophenyl (TNP)-modified murine syngeneic spleen cells. H-2 homology between primary and secondary TNP-modified stimulating cells was required in order to restimulate in the secondary CML. Strong proliferative responses (MLR) were detected only in the secondary cultures, for which H-2 homology was also required between TNP-modified primary and secondary immunogens. Intra-H-2 mapping for the secondary MLR indicated that the relevant regions of homology were I, D, and K and/or I-A. Homology throughout the entire major histocompatibility complex or at K plus I-A gave stronger MLR than did cultures in which there was homology between the primary and secondary phases at I or D only.This publication has 3 references indexed in Scilit:
- Cell-mediated lympholysis to H-2-matched target cells modified with a series of nitrophenyl compounds.The Journal of Experimental Medicine, 1976
- Generation of T Memory Cells in One‐Way Mixed Lymphocyte CultureScandinavian Journal of Immunology, 1976
- Cell-mediated lympholysis of N-(3-nitro-4-hydroxy-5-iodophenylacetyl)-beta-anaylglycylglycyl-modified autologous lymphocytes. Effector cell specificity to modified cell surface components controlled by the H-2K and H-2D serological regions of the murine major histocompatibility complex.The Journal of Experimental Medicine, 1976