Localization of aminopeptidase A (angiotensinase A) in the rat and mouse kidney
- 1 January 1981
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 72 (2), 269-278
- https://doi.org/10.1007/bf00517140
Abstract
Aminopeptidase A (E.C.3.4.11.7; APA) can be demonstrated histochemically in the rat and mouse kidney by light microscopy (simultaneous azo coupling with α-Glu-MNA as substrate and high-purity FBB as coupling agent) mainly in the brush borders, glomeruli and portions of the juxtaglomerular apparatus. Sex and species differences are found with regard to enzyme activity and localization. The relation of aminopeptidase A to angiotensinase A was established by inhibition experiments with angiotensin II and III. The following significant differences exist with respect to other aminopeptidases (aminopeptidase M and γ-glutamyl transferase), which were also demonstrated: APM shows no dependence on calcium ions; APM and γ-GT are not demonstrable in the glomerulus or juxtaglomerular apparatus.This publication has 24 references indexed in Scilit:
- High affinity binding of 125I-angiotensin II to rat glomerular basement membranes.JCI Insight, 1977
- Possible precursors of aminopeptidase and alkaline phosphatase in the proximal tubules of kidney and the crypts of small intestine of miceHistochemistry and Cell Biology, 1974
- Purification of aminopeptidase a in human serum and degradation of angiotensin II by the purified enzymeBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- HISTOCHEMICAL AND ULTRASTRUCTURAL DEMONSTRATION OF γ-GLUTAMYL TRANSPEPTIDASE ACTIVITYJournal of Histochemistry & Cytochemistry, 1969
- Removal of Angiotensin by Isolated Perfused Organs of the RatNature, 1969
- Observations on the segmentation of the proximal tubule in the rat kidneyJournal of Ultrastructure Research, 1966
- Arylamidase activity related to angiotensinaseBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1965
- HISTOCHEMICAL DEMONSTRATION OF A GAMMA-GLUTAMYL TRANSPEPTIDASE-LIKE ACTIVITYJournal of Histochemistry & Cytochemistry, 1962
- A Mammalian Peptidase specific for the Hydrolysis of N-Terminal α-L-Glutamyl and Aspartyl ResiduesNature, 1962
- Glutamyl Peptidases in Rat and Guinea Pig Kidney SlicesNature, 1961