Activation and Tuning of Green Fluorescent Protein Chromophore Emission by Alkyl Substituent-Mediated Crystal Packing
- 23 December 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (2), 662-670
- https://doi.org/10.1021/ja806962e
Abstract
O-Alkyl synthetic analogues of the green fluorescent protein chromophore are nonfluorescent in solution but demonstrate a bright emission in the crystalline state. Three-dimensional steady-state and time-resolved emission spectroscopies in the solid state, as well as single-crystal X-ray diffraction, reveal the nature of complex emission in the crystals. A hypsochromic emission shift with an increase of the alkyl size is determined by the monomer-aggregate emission ratio.Keywords
This publication has 74 references indexed in Scilit:
- Photonics and photochemical stability of aromatic molecules, family related in π-structure but different in planarity, rigidity and molecule symmetryJournal of Photochemistry and Photobiology A: Chemistry, 2008
- Multivariate methods on the excitation emission matrix fluorescence spectroscopic data of diesel–kerosene mixtures: A comparative studyAnalytica Chimica Acta, 2007
- The Gas-Phase Absorption Spectrum of a Neutral GFP Model ChromophoreBiophysical Journal, 2007
- J-Aggregation in α-Sexithiophene Submonolayer Films on Silicon DioxideJournal of the American Chemical Society, 2006
- The Role of the Protein Matrix in Green Fluorescent Protein FluorescencePhotochemistry and Photobiology, 2006
- Solid-state optical properties of linear polyconjugated molecules: π-stack contra herringboneThe Journal of Chemical Physics, 2005
- Expanded dynamic range of fluorescent indicators for Ca 2+ by circularly permuted yellow fluorescent proteinsProceedings of the National Academy of Sciences, 2004
- Intermolecular Interactions in π-Stacked Conjugated Molecules. Synthesis, Structure, and Spectral Characterization of Alkyl Bithiazole OligomersJournal of the American Chemical Society, 2003
- The fundamental photophysics of conjugated oligomer herringbone aggregatesThe Journal of Chemical Physics, 2003
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998