EXTRACTION OF ABSORBED LIPID (LINOLEIC ACID-1-14C) FROM RAT INTESTINAL EPITHELIUM DURING PROCESSING FOR ELECTRON MICROSCOPY
Open Access
- 1 July 1968
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 38 (1), 252-255
- https://doi.org/10.1083/jcb.38.1.252
Abstract
Everted sacs of rat jejunum were incubated in a bile salt solution of linoleic acid-1 C14. After a 30-min. absorptive period the tissue was fixed, dehydrated, and embedded for electron microscopy. Analysis of the solutions used in tissue preparation showed that 26 [degree]/o of the absorbed lipid was lost to the solutions. Preliminary studies reported support the possibility that lipid absorbed into the microvilli and terminal web of intestinal epithelial cells is significantly extracted during tissue preparation for electron microscopy.This publication has 15 references indexed in Scilit:
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