Conjugation of N-Allylnormorphine by Liver Slices

Abstract

N-allylnormorphine can be estimated by use of the Snell and Snell reagent, for a change in the alkyl group on the N does not change the intensity of the color produced. N-allylnormorphine is conjugated by surviving liver slices at the same rate as is morphine. In 2.5 hours, about 75% of the 2 compounds is conjugated. Because conjugation of the 2 compounds proceeds at essentially the same rate and the effective blocking dose of N-allylnormorphine is small, the latter compound must be more firmly fixed to the receptor site than is morphine.