Yeast mating pheromone alpha factor inhibits adenylate cyclase.
- 1 April 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (4), 1898-1902
- https://doi.org/10.1073/pnas.77.4.1898
Abstract
The pheromone .alpha. factor, secreted by S. cerevisiae cells of the .alpha. mating type, serves to synchronize the opposite mating type (a cells) at G1 as a prelude to fusion of the 2 cell types. In vitro, .alpha. factor inhibited the membrane-bound adenylate cyclase of these cells in a dose-dependent manner. Moreover, one class (ste5) of a cell mutants that grow normally at either 23.degree. or 34.degree. C but that are unable to respond to .alpha. factor or to mate at the higher temperature possessed an adenylate cyclase activity that was not inhibited by .alpha. factor at 34.degree. C but was fully sensitive to inhibition at 23.degree. C. Furthermore, addition of cyclic AMP to a cell culture medium shortened the period of pheromone-induced G1 arrest. Inhibition of adenylate cyclase activity by .alpha. factor may constitute, at least in part, the biochemical mode of action of the pheromone in vivo.This publication has 29 references indexed in Scilit:
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