Modular electronics for flow cytometry and sorting: The LACEL system

Abstract

LACEL is a newly developed, fast, general-purpose data acquisition and processing system for flow cytometric applications. The system's modular electronics allows flexibility in system configurations. The system can process as many as eight input analog parameters and can transfer 16-bit words between the user's electronics and the computer with standard input/output interfaces. The system's 8-fold coincidence logic capability can be set to operate with the noncoincidental timing that may occur in multiparameter flow measurements. As many as four parameters can be used to establish amplitude and timing criteria for each of two sorting directions. Two experiments can be on line with the computer at one time.