A Study of Calf‐Thymus Histone H2B Using 13C Magnetic Resonance
- 1 November 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 70 (2), 403-408
- https://doi.org/10.1111/j.1432-1033.1976.tb11030.x
Abstract
Calf thymus histone H2B was studied by means of 13C magnetic resonance. Most side-chain resonances were assigned. Analysis of 13C peak intensities by a simple computer-aided method proves that 13C magnetic resonance spectroscopy can be used to monitor aggregation phenomena more accurately than 1H magnetic resonance or other physico-chemical methods. Re-examination of 1H spectral simulations shows substantial agreement with 13C results. In 0.1 M added NaCl the H2B aggregates probably include the chain segment from 50 (.+-. 5) to 108 (.+-. 5).This publication has 12 references indexed in Scilit:
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