Tetrahedral Intermediate in a Specific α-Chymotrypsin Inhibitor Complex Detected by Laser Raman Spectroscopy
- 1 August 1975
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 189 (4200), 384-386
- https://doi.org/10.1126/science.238285
Abstract
Laser Raman spectroscopy indicates that inhibition of alpha-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.Keywords
This publication has 14 references indexed in Scilit:
- New, specific and reversible bifunctional alkylborinic acid inhibitor of acetylcholinesteraseBiochemistry, 1974
- Determination of dissociation constants of crystalline α-chymotrypsin complexesJournal of Molecular Biology, 1974
- Search for the addition of monodentate nucleophiles to boric and benzeneboronic acids in waterThe Journal of Organic Chemistry, 1972
- 2-Phenylethaneboronic acid, a possible transition-state analog for chymotrypsinBiochemistry, 1971
- Laser-excited Raman spectroscopy of biomolecules: II. Native ribonuclease and α-chymotrypsinJournal of Molecular Biology, 1970
- n‐Alkylboronic acids as bifunctional reversible inhibitors of α‐chymotrypsinFEBS Letters, 1970
- Structure of crystalline α-chymotrypsinJournal of Molecular Biology, 1969
- Structure of crystalline α-chymotrypsinJournal of Molecular Biology, 1968
- Boric acid esters. I. General survey of aromatic ligands and the kinetics and mechanism of the formation and hydrolysis of boric acid esters of salicylamide, N-phenylsalicylamide, and disalicylimideJournal of the American Chemical Society, 1967
- An X-ray diffraction study of inhibited derivatives of α-chymotrypsinJournal of Molecular Biology, 1966