Isolation from corynebacterium diphtheriae C7(beta) of bacterial mutants that produce toxin in medium with excess iron
- 1 October 1977
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 18 (1), 203-209
- https://doi.org/10.1128/iai.18.1.203-209.1977
Abstract
Five mutants that produce toxin in medium with excess Fe were isolated from strain C7(.beta.). The Fe content of bacteria grown on this medium was considerably higher than that of C7(.beta.) cells grown in medium containing the minimum amount of Fe needed to inhibit toxin production. When the nonlysogenic, nontoxinogenic strain C7(-) was lysogenized with phages from each of the mutants, toxin production by all of the resulting lysogens, like that by parent strain C7(.beta.), ceased upon Fe addition. When the mutants were superinfected with .beta.45 phage, both toxin and CRM45 [45,000 MW cross reacting material] were produced in medium with excess Fe. One of the mutant strains lost its prophage as a result of treatment with UV light. When the cured strain was lysogenized with phage carrying a mutation in the tox structural gene, the lysogen produced the mutant protein at the maximum rate in medium with excess Fe. Evidently the mutant strains are not phage mutants, but are bacterial host mutants, and a host factor(s) is involved in inhibition of toxin production by Fe.This publication has 23 references indexed in Scilit:
- Synthesis of Diphtheria tox -Gene Products in Escherichia coli ExtractsProceedings of the National Academy of Sciences, 1974
- Expression of diphtheria toxin genes carried by integrated and nonintegrated phage betaVirology, 1972
- Reconstitution of Diphtheria Toxin from Two Nontoxic Cross-Reacting Mutant ProteinsScience, 1972
- A phage-mutant directed synthesis of a fragment of diphtheria toxin proteinBiochemical and Biophysical Research Communications, 1972
- Mutation in the Structural Gene for Diphtheria Toxin carried by Temperate Phage βNature New Biology, 1971
- The Distribution of Iron in Iron-deficient Toxin-synthesizing and in Excess-iron Non-toxin-synthesizing Corynebacterium DiphtheriaeJournal of General Microbiology, 1969
- Phage-host relationships in certain strains of Corynebacterium diphtheriaeVirology, 1966
- Phage-Directed Synthesis of Diphtherial Toxin in Non-Toxinogenic Corynebacterium diphtheriaeNature, 1966
- Kinetics of Diphtheria Toxin FormationJournal of General Microbiology, 1962
- The Uptake of Iron by Corynebacterium diphtheriae Growing in Submerged CultureJournal of General Microbiology, 1960