Studies of lipid A fractions from the lipopolysaccharides of Pseudomonas aeruginosa and Pseudomonas alcaligenes
- 1 July 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 133 (3), 563-572
- https://doi.org/10.1042/bj1330563
Abstract
Lipid A fractions from Pseudomonas aeruginosa and Pseudomonas alcaligenes have similar compositions and structural features. By means of hydrazinolysis of the parent lipopolysaccharides and partial hydrolysis of the deacylation products, it was established that both lipids are derived from the β-(1→6)-linked disaccharide of glucosamine. Phosphorylated derivatives of the disaccharide from Ps. aeruginosa were also characterized. The lipids differ mainly in the absence of hexadecanoic acid and 2-hydroxydodecanoic acid from the lipid from Ps. alcaligenes. Evidence that in Ps. aeruginosa these acids are ester-linked to residues of 3-hydroxyalkanoic acids (including 3-hydroxydecanoic acid) was obtained. Heterogeneity of lipid A fractions was indicated by t.l.c., and by gel filtration of de-O-acylation products from mild alkaline methanolysis of the lipids.Keywords
This publication has 28 references indexed in Scilit:
- Cell Walls, Lipids, and Lipopolysaccharides of Pseudomonas SpeciesEuropean Journal of Biochemistry, 1973
- Nature and Linkages of the Fatty Acids Present in the Lipid‐A Component of Salmonella LipopolysaccharidesEuropean Journal of Biochemistry, 1972
- Evidence for two regions in the polysaccharide moiety of the lipopolysaccharide of Pseudomonas aeruginosa 8602FEBS Letters, 1970
- Structural features of lipid A preparations isolated from Escherichia coli and Shigella flexneriBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970
- The chemical composition of cell-wall lipopolysaccharides from Moraxella duplex and Micrococcus calco-aceticusCanadian Journal of Microbiology, 1970
- The linkage between the d-glucosamine units of lipid A isolated from the lipopolysaccharide of Serratia marcescensBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1969
- Cell wall lipopolysaccharides from Neisseria catarrhalisCanadian Journal of Microbiology, 1969
- Biochemical Studies on Lipopolysaccharides of Salmonella R MutantsEuropean Journal of Biochemistry, 1969
- Detection of Sugars on Paper ChromatogramsNature, 1950
- Separation of the Phosphoric Esters on the Filter Paper ChromatogramNature, 1949