Organization of intramembrane particles in freeze-cleaved gap junctions of rat graafian follicles: optical-diffraction analysis

Abstract

Gap junctions were identified in the membrana granulosa and cumulus oophorus, and between cells of the internal theca, of the preovulatory rat follicle. In replicas of freeze fractured follicles, the A face presented clusters of closely packed intramembrane particles, 7—9 nm in diameter, forming a mosaic pattern, while the B face showed a similar pattern of small pits. Optical diffraction analysis of these electron micrographs revealed that both the intramembrane particles and the corresponding pits were organized in hexagonal lattices with centre-to-centre spacing of 9—10 nm. In small junctions (up to 0·4 μm in diameter), both A and B faces generally consisted of a single lattice, while large junctions (0·5–2 μm) contained multiple lattices differing in orientation. Occasionally intramembrane particles and pits were more loosely arranged throughout the junctional area and failed to show a hexagonal pattern. Both granulosa and thecal cells often contained intracellular vesicles resembling annular junctions. These contained intramembrane particles whose assembly resembled that of the gap junctions with regard to periodicity and lattice organization. Examination of thin sections suggested that small gap junctions occur also between cytoplasmic processes of coronal cells and the oolemma. No tight junctions were detected between granulosa cells and between thecal cells.