Copenhagen rat prostatic tumor ornithine decarboxylase activity (ODC) and the effect of the ODC inhibitor alpha-difluoromethylornithine
- 1 January 1982
- journal article
- research article
- Published by Wiley in The Prostate
- Vol. 3 (4), 383-389
- https://doi.org/10.1002/pros.2990030408
Abstract
The R3327MAT-Lu tumor is a rapidly growing anaplastic derivative of the Dunning R3327 prostatic adenocarcinoma. We have found the ornithine decarboxylase (ODC) activity of this tumor to be as sensitive to inhibition by alpha-difluoromethylornithine (DFMO) as normal rat prostate. The same was true for all the other R3327 tumor derivatives we studied. The in vivo inhibition of ODC by DFMO allowed increased uptake of exogenously administered putrescine by the R3327 AT tumor. Further, DFMO was inhibitory to the growth of the R3327MAT-Lu both in vitro and in vivo.Keywords
This publication has 12 references indexed in Scilit:
- Synergistic action of two polyamine antimetabolites leads to a rapid therapeutic response in childhood leukemiaInternational Journal of Cancer, 1981
- Aminoguanidine reversal of the inhibitory effects of ornithine analogs on the in vitro clonogenic survival of the R3327 AT prostate-derived tumorCancer Letters, 1981
- Intracellular putrescine and spermidine deprivation induces increased uptake of the natural polyamines and methylglyoxal bis(guanylhydrazone)Biochemical Journal, 1980
- Effect of α-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ornithine decarboxylase, on polyamine levels in rat tissuesLife Sciences, 1979
- A microprocedure for the measurement of 14CO2 release from [14C]carboxyl-labeled amino acidsAnalytical Biochemistry, 1978
- Pyridoxal 5′‐Phosphate and the Regulation of Ornithine Decarboxylase Activity and StabilityEuropean Journal of Biochemistry, 1976
- 1,4-Diaminobutane (Putrescine), Spermidine, and SpermineAnnual Review of Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Ornithine decarboxylase activity and: [125I]iododeoxyuridine incorporation in rat prostateBiochemical Journal, 1975