Molecular cloning of human prostate specific antigen cDNA
- 20 April 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 214 (2), 317-322
- https://doi.org/10.1016/0014-5793(87)80078-9
Abstract
A λgt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library. The cDNA insert of 1415 nucleotides hybridizes specifically to a prostate mRNA species of 1.5 kb. The nucleotide sequence codes for part of a signal peptide, a short propiece and the mature protein of 237 amino acid residues. The M r for the non-glycosylated protein was 26 089. One potential site for N-linked carbohydrate attachment was identified. The primary structure shows extensive homology with proteases of the kallikrein family.Keywords
This publication has 24 references indexed in Scilit:
- Nucleotide sequence of cloned cDNA for human pancreatic kallikreinBiochemistry, 1985
- A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein.Journal of Clinical Investigation, 1985
- Tissue-specific expression of kallikrein-related genes in the ratBiochemistry, 1985
- Amino acid sequence of the predominant basic protein in human seminal plasmaFEBS Letters, 1985
- Yeast RNA Polymerase II Genes: Isolation with Antibody ProbesScience, 1983
- Structure of mouse kallikrein gene family suggests a role in specific processing of biologically active peptidesNature, 1983
- Refined 2 Å X-ray crystal structure of porcine pancreatic kallikrein A, a specific trypsin-like serine proteinaseJournal of Molecular Biology, 1983
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexaminationBiochemistry, 1977